Gareth Bale is not available for Wales' World Cup Qualifiers in October due to a "significant" hamstring injury he suffered after the previous international break.
The Real Madrid man has not played since Wales' 0-0 draw with Estonia on September 8 having picked up the injury after returning to his club.
The injury could keep out until late November, after his country's last two qualifiers against Belarus and Belgium, meaning his wait for his 100th cap goes on.
Bale could have made the milestone appearance against Czech Republic next month but his injury has seen him not included in Robert Page's squad.
Wales face the Czech Republic and Estonia in October as they look to qualify for their first World Cup since 1958.
They are currently in third place in group E, level on points with second place Czech Republic but have played one less than their rivals.
Page will be without his talismanic forward for at least the next two matches and is concerned he could be out for longer.
"You grade hamstring injuries from one to four and his is nearer the four," stated the Wales boss.
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"So it's quite a significant hamstring tear. We've been in close contact with him to see how it's progressing but this camp has come too soon for him.
"Originally it might have been progressing into November's camp, that's the significance of the injury.
"Thankfully he's recovering better than anticipated. We're pretty certain we'll have him for November."
Bale had started Real Madrid's first three matches of the season before picking up the injury, having returned to the club following his loan spell at Tottenham Hotspur last season.
This is the latest in a string of injuries that have disrupted his eight years in Spain, as he will now have missed 109 Real Madrid matches through injury since 2013 if he is sidelined until November.
Page went on to say of the injury: "Whether it was to do with the travelling in September's camp and he had a build-up in fatigue in his muscles and a soft tissue injury… it's a blow.
"Gareth is a very special player and we know he will be missed. But we can't cry about it, we've got to get on with it and look at it in a positive frame of mind."
Elsewhere, Aaron Ramsey has been included in Page's 28-man squad, having been one of a number of withdrawals from the previous selection.
While Huddersfield Town winger Sorba Thomas has been handed his first call-up after a strong start to the season that saw him win the Championship's August Player of the Month.
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